Leica LMD 7000

Leica LMD 7000 Instrument

The Leica LMD7000 Laser Microdissection System is an automated upright microscope platform that uses a 349 nm solid-state diode UV laser to isolate target areas from biological tissues, plant materials, and cell cultures. It utilizes integrated brightfield, DIC, and fluorescence imaging alongside gravity-based sample collection into standard microfuge caps for downstream molecular analysis.

Core System Features

  • Laser System: 349 nm solid-state diode UV laser with 10–5,000 Hz adjustable pulse frequency and up to 120 µJ maximum energy.
  • Optical Platform: Automated upright research microscope with dedicated dry objectives ranging from 1.25x to 150x.Imaging Modes: Integrated brightfield, Differential Interference Contrast (DIC), and fluorescence capabilities with BGR and GFP filter cubes.
  • Imaging Modes: Integrated brightfield, Differential Interference Contrast (DIC), and fluorescence capabilities with BGR and GFP filter cubes.
  • Collection Method: Gravity-based contact-free collection into standard 0.2 mL microfuge caps, 0.5 mL microfuge caps, or 8-strip PCR wells.
  • Motorized Condenser: Features an automated universal condenser that dynamically adjusts to match the optimal settings for the 10x, 20x, 40x, or 63x objectives for optimized contrast and resolution.
  • Sample Compatibility: Sectioning capabilities for frozen tissues, formalin-fixed paraffin-embedded (FFPE) tissues, plant materials, and live cell cultures.
  • Software Automation: Real-time drawing and cutting, automated batch-cutting via pattern recognition, and 2.5x whole-slide scanning for overview mapping.
  • Downstream Alignment: Isolation of targeted materials for DNA/RNA sequencing, qRT-PCR, proteomics, mass spectrometry, and metabolomics.